No any step like blocking is involved in southern blotting.ĭuring western blotting, nonspecific antibody sites are blocked on the nitrocellulose paper with the help of milk powder or bovine serum albumin (BSA).Ĭommon labeling methods that are used in southern blotting are the use of chromogenic dyes or radiolabelling or fluorescent labeling etc. Western Blotting follows electric transfer procedure.ĭuring the Southern blotting, the sample needs to be denaturated.ĭuring western blotting, the sample should be in its native state. Southern blotting follows capillary transfer procedure. SDS PAGE/ Polyacrylamide gel used to separate proteins in western blotting Primary and secondary antibodies are used as a probe.Īgarose gel electrophoresis is used in Southern blotting. It works on the principle of immunodetection method or antigen-antibody interaction.Ī single-stranded DNA or sometimes RNA is used as a probe. It works on the principle of hybridization. Western blotting is used to find out a specific amino acid or protein sequence. Southern blotting is used to find out a specific DNA sequence. Western blotting was developed by George Stark’s group at Stanford University in 1979. Southern blotting was developed by Edward M. ADVERTISEMENT Comparison Chart Southern BlottingĪ technique that is used to find out a specific DNA sequence in a given mixture is known as southern blotting.Ī technique that is used to find out a specific amino acids sequence of the protein in a given mixture is known as southern blotting.
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